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Microbiology JoVE (Open Access) Citable · DOI

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Name: Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Uploaded: 2026-10-13
Duration: 10 min 18 s
Description: Learn to amplify near full-length HIV-1 proviruses from single infected cells using nested PCR for next-generation sequencing. This FLIPS method enables identification of replication-competent proviruses.

DOI: 10.3791/58016-v

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