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Analytical Chem JoVE (Open Access) Citable · DOI

An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation

DOI: 10.3791/54765-v
What you'll learn
  • ✓ Construct a helium-operated kHz frequency plasma jet apparatus
  • ✓ Design and build a controlled reactor to isolate plasma reactive species
  • ✓ Measure reactive oxygen species formation without atmospheric interference
  • ✓ Analyze and interpret plasma-induced chemistry data
Protocol

Biopharma Insights An experimental setup was created for the helium-operated kHz frequency plasma jet. The setup includes a cage for the plasma power supply and jet and an in-house built reactor to monitor plasma-induced reactive species without the interference of the ambient atmosphere.

Difficulty
advanced
Total time
~4-6 hours (setup and calibration); reactive species measurement ~1-2 hours per experimental run

Steps

1
Construct the atmospheric pressure plasma jet

Assemble the helium-operated kHz frequency plasma jet apparatus, including the power supply cage and jet delivery system according to specifications.

▶ 01:05
2
Build isolated reactor for reactive species monitoring

Construct the in-house built reactor vessel designed to exclude ambient atmosphere and enable controlled measurement of plasma-induced reactive species formation.

▶ 03:36
3
Analyze reactive oxygen species formation and composition

Measure and characterize reactive oxygen species generated by the plasma system using analytical methods with atmospheric interference eliminated.

▶ 06:13
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