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Neuroscience JoVE (Open Access) Citable · DOI

An Experimental Platform to Study the Closed-loop Performance of Brain-machine Interfaces

DOI: 10.3791/1677-v
What you'll learn
  • ✓ Set up and calibrate a closed-loop fly-machine interface system
  • ✓ Perform fly preparation and electrode positioning for neural recordings
  • ✓ Conduct visual stimulation experiments while recording neuronal activity
  • ✓ Analyze closed-loop performance data from a behaving model organism
Protocol

Biopharma Insights We use a closed-loop fly-machine interface to investigate general principles in neuronal control.

Difficulty
advanced
Total time
~2–3 hours per fly (including preparation, positioning, and recording session)
Model organism
Drosophila melanogaster (fruit fly)

Steps

1
Understand closed-loop brain-machine interface principles

Review the conceptual framework and experimental rationale for investigating neuronal control using a fly-machine interface system.

▶ 00:47
2
Prepare and mount the fly specimen

Anesthetize, position, and secure the fly in the experimental apparatus with proper immobilization for neural recording.

▶ 01:21
3
Position recording electrode over target brain region

Locate and advance the microelectrode to the desired neural site using visual guidance and stereotaxic alignment.

▶ 03:58
4
Deliver visual stimuli and record neural signals

Present controlled visual stimuli while simultaneously recording extracellular or intracellular neuronal activity in closed-loop mode.

▶ 04:48
5
Analyze and interpret representative experimental results

Review neural response data, behavioral correlates, and closed-loop feedback performance metrics from the recording session.

▶ 07:18
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