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Botany JoVE (Open Access) Citable · DOI

An Implantable System For Chronic In Vivo Electromyography

DOI: 10.3791/60345-v
What you'll learn
  • ✓ Manufacture bipolar stimulus electrode cuffs for laryngeal nerves
  • ✓ Prepare chronically implantable EMG recording electrodes for muscle monitoring
  • ✓ Perform surgical implantation of chronic electromyography system in animal models
  • ✓ Acquire and interpret evoked and spontaneous laryngeal muscle potentials
Protocol

Presented here is a protocol for the manufacturing of an implantable system for in vivo chronological recording of evoked and spontaneous electromyographic potentials. The system is applied to the investigation of reinnervation of laryngeal muscles following nerve injury.

Difficulty
advanced
Total time
~4–6 hours per animal (surgical implantation + electrode preparation)
Model organism
Canine (dog)

Steps

1
Prepare bipolar RLN and SLN stimulus electrode cuffs

Fabricate electrode cuff devices for stimulation of recurrent and superior laryngeal nerves. This foundational step enables nerve-evoked muscle response recording.

▶ 01:11
2
Prepare PCA muscle EMG recording electrodes

Construct implantable recording electrodes for posterior cricoarytenoid muscle electromyography measurements.

▶ 03:45
3
Prepare skin receptacle connector system

Assemble subcutaneous connector port for chronic percutaneous access to electrode leads during longitudinal recordings.

▶ 04:18
4
Implant chronic electromyography system surgically

Execute surgical placement of stimulus cuffs, recording electrodes, and skin receptacle in animal model. Demonstration uses canine cadaver.

▶ 06:13
5
Acquire representative EMG recordings from normal innervation

Obtain baseline evoked and spontaneous electromyographic potentials from properly innervated laryngeal muscles to establish normal response patterns.

▶ 08:18
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