Cell Biology JoVE (Open Access) Citable · DOI

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

Name: Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
Uploaded: 2026-09-04
Duration: 7 min 34 s
Description: The zebrafish model is an emerging system for the study of neuromuscular disorders. In the study of neuromuscular diseases, the integrity of the muscle ...

DOI: 10.3791/53183-v

What you'll learn

✓ Prepare agar injection plates for zebrafish larvae
✓ Mix and load Evans Blue Dye injection solution
✓ Perform pericardial injection in zebrafish larvae
✓ Assess skeletal muscle integrity via EBD uptake

Protocol

Biopharma Insights In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.

Difficulty

intermediate

Total time

~2-3 hrs/session (plus overnight incubation)

Model organism

Zebrafish (Danio rerio) larvae

Biosafety

BSL-1

Steps

Prepare agar plates and EBD injection mix

Cast agar injection plates and prepare the Evans Blue Dye injection mixture for larval injections.

▶ 01:14

Prepare Evans Blue Dye injection solution

Load the Evans Blue Dye solution into the injection needle, ready for pericardial delivery.

▶ 02:42

Inject EBD into larval pericardium

Perform pericardial injection of Evans Blue Dye and allow uptake in zebrafish larvae.

▶ 03:54

Analyze skeletal muscle integrity

Examine Evans Blue Dye uptake to assess skeletal muscle integrity in zebrafish larvae.

▶ 05:29

Conclude EBD muscle analysis

Summarize the EBD method for characterizing muscle integrity and dystrophy models.

▶ 06:14

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