This protocol describes a full kidney work-up that should be carried out in mouse models of glomerular disease. The methods allow for detailed functional, structural, and mechanistic analysis of glomerular function, which can be applied to all mouse models of glomerular disease.
Total time
~3-4 days (tissue collection, processing, staining, and analysis)
Model organism
Mouse (unspecified strain, applicable to glomerular disease models)
Steps
1
Collect and prepare kidney samples
Harvest kidney tissue from experimental mice and prepare samples for downstream functional and structural analysis. This foundational step ensures proper tissue quality for subsequent assays.
▶ 00:30
2
Isolate glomeruli and measure water permeability
Perform mechanical isolation of glomeruli from kidney tissue and quantify glomerular water permeability (LpA/Vi) using standardized methods to assess functional filtration capacity.
▶ 03:27
3
Perform PAS staining on kidney tissue
Apply Periodic Acid Schiff staining to kidney sections to visualize and assess structural changes in glomeruli and basement membranes characteristic of glomerular disease.
▶ 05:36
4
Analyze and interpret kidney function results
Review representative data from functional and histological analyses to demonstrate integration of kidney function assessment outcomes in glomerular disease models.
▶ 06:55