Home › Botany › Assessment of Methane and Nitrous Oxide Fluxes from Paddy Field by Means of Static Closed Chambers Maintaining Plants Within Headspace
Botany JoVE (Open Access) Citable · DOI

Assessment of Methane and Nitrous Oxide Fluxes from Paddy Field by Means of Static Closed Chambers Maintaining Plants Within Headspace

DOI: 10.3791/56754-v
What you'll learn
  • ✓ Design and construct static closed chambers for paddy field gas sampling
  • ✓ Measure CH₄ and N₂O fluxes while maintaining living plants in headspace
  • ✓ Collect, handle, and analyze gas samples from flooded agricultural systems
  • ✓ Interpret seasonal greenhouse gas emission patterns from rice paddies
Protocol

The overall goal of this protocol is to measure greenhouse gas emissions from paddy fields using the static closed chamber technique. The measurement system needs specific adjustments due to the presence of both a permanent water layer in the field and of the plants within the chamber headspace.

Difficulty
advanced
Total time
~30 min per chamber deployment; full seasonal study requires repeated measurements over 12 months

Steps

1
Design static closed chamber for paddy fields

Construct chamber apparatus with modifications to accommodate permanent water layer and plant growth within headspace. Ensure proper sealing and gas-tight design for accurate flux measurements.

▶ 01:13
2
Close chamber and collect greenhouse gas samples

Deploy closed chamber system in field, obtain gas samples from headspace at defined time intervals, and perform initial handling protocols for CH₄ and N₂O quantification.

▶ 03:44
3
Analyze CH₄ and N₂O concentrations and calculate fluxes

Process collected samples using chromatography or spectroscopy; calculate gas flux rates from concentration changes over time and chamber geometry to determine seasonal emission patterns.

▶ 06:46
💬 Comments coming soon