✓Set up Crawley's sociability and social novelty test apparatus
✓Conduct social interaction trials in mice
✓Interpret sociability indices from test results
✓Compare social behavior between wild-type and knockout mice
Protocol
Biopharma Insights Here we describe a detailed protocol for examination of sociability in mice by using Crawley's sociability and preference for social novelty test. We describe the advantages and possible applications for this procedure, including critical details important for correct interpretation of the results.
Difficulty
intermediate
Total time
~30-45 min/mouse across multiple sessions
Model organism
Mouse C57BL/6J
Biosafety
BSL-1
Steps
1
Prepare equipment and testing room
Arrange the three-chambered apparatus and ensure proper lighting, temperature, and habituation conditions for behavioral testing.
▶ 01:24
2
Perform social interaction trials
Place subject mouse in the apparatus and record interactions with a stranger mouse versus an empty chamber or object.
▶ 02:23
3
Analyze knockout versus wild-type behavior
Compare sociability and social novelty preference between GSK-3α knockout mice and wild-type controls using recorded data.
▶ 04:54
4
Interpret results and conclude
Summarize findings on social behavior differences and consider implications for the experimental model.
▶ 06:08
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