Bioluminescence imaging is a well-known tool for localizing tumors and metastases, but quantification of these images often requires complex calculations and particular instruments. We describe the easy-to-use luminoscore method, based on precise acquisition conditions, requiring no calculations, and enabling tumor burden and treatment response to be monitored in mouse models.
Total time
~15-30 min per imaging session; varies by tumor progression timeline (days to weeks)
Model organism
Mouse (lymphoma xenograft/transgenic models)
Steps
1
Set up bioluminescence imaging system
Configure imaging equipment with precise acquisition parameters to ensure consistent, standardized conditions for reproducible bioluminescence signal detection and quantification.
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2
Quantify bioluminescence signal using luminoscore
Apply the luminoscore method to acquired images, converting raw bioluminescence data into tumor burden measurements without requiring manual calculations or specialized analysis software.
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3
Monitor tumor burden and treatment response
Track bioluminescence quantification data over time in mouse lymphoma models to assess tumor progression and evaluate the efficacy of therapeutic interventions.
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