Home › Cell Biology › Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization
Cell Biology JoVE (Open Access) Citable · DOI

Bioprinting of Cartilage and Skin Tissue Analogs Utilizing a Novel Passive Mixing Unit Technique for Bioink Precellularization

DOI: 10.3791/56372-v
What you'll learn
  • ✓ Prepare nanocellulose-alginate bioink and cellularize via passive mixing
  • ✓ Execute bioprinting protocol for cartilage and skin tissue analogs
  • ✓ Assess cell viability and differentiation markers in bioprinted constructs
Protocol

Cartilage and skin analogs were bioprinted using a nanocellulose-alginate based bioink. The bioinks were cellularized prior to printing via a single step passive mixing unit. The constructs were demonstrated to be uniformly cellularized, have high viability, and exhibit favorable markers of differentiation.

Difficulty
advanced
Total time
~3–5 days (including cell preparation, mixing, printing, and culture/analysis)
Model organism
Primary cells or cell lines (species-dependent; human or animal-derived)
Biosafety
BSL-1

Steps

1
Prepare consumables, bioink, and cell suspension

Prepare nanocellulose-alginate bioink and cell suspension according to protocol specifications. Ready all consumables and equipment needed for the mixing and printing workflow.

▶ 00:51
2
Mix cell suspension with bioink using passive unit

Combine cell suspension and bioink in a single-step passive mixing unit to achieve uniform cellularization prior to printing.

▶ 02:33
3
Bioprint cartilage analogs with single cell type

Load cellularized bioink into the bioprinter and execute the printing protocol to generate cartilage tissue analog constructs.

▶ 04:44
4
Assess cell viability and long-term culture performance

Evaluate cell viability immediately after mixing and monitor differentiation markers throughout culture period to confirm construct quality.

▶ 07:28
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