Here, we present a protocol to take blood samples from the subclavian vein of mice.
Assemble all necessary supplies and equipment required for subclavian vein puncture before beginning the procedure.
Restrain and position the mouse supine or laterally to expose the subclavian vein region for puncture.
Locate and puncture the subclavian vein using proper technique to obtain blood sample while minimizing tissue damage.
Verify successful collection, assess sample quality, and document results from the subclavian vein puncture.
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