Home › Neuroscience › Brain Source Imaging in Preclinical Rat Models of Focal Epilepsy using High-Resolution EEG Recordings
Neuroscience JoVE (Open Access) Citable · DOI

Brain Source Imaging in Preclinical Rat Models of Focal Epilepsy using High-Resolution EEG Recordings

DOI: 10.3791/52700-v
What you'll learn
  • ✓ Prepare and secure high-resolution EEG mini-cap on sedated rats
  • ✓ Conduct noninvasive EEG recordings from focal epilepsy models
  • ✓ Perform brain source imaging analysis from interictal epileptiform discharges
  • ✓ Localize epileptic foci using volume conductor modeling
Protocol

This video introduces the preparation, recording, and source analysis procedures of high-resolution EEG on sedated rats with a particular preclinical model of focal epilepsy under noninvasive conditions.

Difficulty
advanced
Total time
~3–4 hours per animal (including sedation, cap placement, recording, and source analysis)
Model organism
Rat (Sprague Dawley or Lewis, preclinical focal epilepsy model)
Biosafety
BSL-1

Steps

1
Prepare EEG mini-cap and animal setup

Assemble the high-resolution EEG mini-cap and prepare the sedated rat for electrode placement. Ensure proper positioning and cap securing on the animal's head for stable recordings.

▶ 02:24
2
Conduct high-resolution EEG recording

Execute noninvasive EEG recording from the sedated focal epilepsy model rat. Capture baseline activity and interictal epileptiform discharges under standardized conditions.

▶ 03:49
3
Build volume conductor head model

Construct a subject-specific or template-based volume conductor model to account for tissue conductivity and electrode-to-source geometry in source localization.

▶ 05:09
4
Perform brain source imaging analysis

Apply source imaging algorithms to reconstruct intracranial sources of recorded epileptiform activity using the volume conductor model and EEG data.

▶ 06:40
5
Localize epileptic foci by spike clustering

Classify interictal epileptiform discharges into clusters and map their brain source locations to identify the focal epilepsy origin zone.

▶ 07:24
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