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Cell Biology JoVE (Open Access) Citable · DOI

Bridging the Bio-Electronic Interface with Biofabrication

DOI: 10.3791/4231-v
What you'll learn
  • ✓ Electrodeposit stimuli-responsive polysaccharide films on biased electrodes
  • ✓ Coculture cells within alginate and chitosan matrices for bioelectronic applications
  • ✓ Functionalize biocompatible films with proteins using enzymatic assembly
  • ✓ Integrate biofunctionalized surfaces into lab-on-chip device platforms
Protocol

This article describes a biofabrication approach: deposition of stimuli-responsive polysaccharides in the presence of biased electrodes to create biocompatible films which can be functionalized with cells or proteins. We demonstrate a bench-top strategy for the generation of the films as well as their basic uses for creating interactive biofunctionalized surfaces for lab-on-a-chip applications.

Difficulty
advanced
Total time
~4-6 hours per film set (electrodeposition, functionalization, characterization)
Biosafety
BSL-1

Steps

1
Electrodeposit alginate films on biased electrodes

Apply electrochemical bias to electrodes in alginate solution to trigger polysaccharide deposition and film formation on the electrode surface.

▶ 02:57
2
Coculture communication cells within alginate matrix

Incorporate live cells into the alginate film during electrodeposition to create interactive cell-laden biocompatible matrices.

▶ 04:40
3
Electrodeposit chitosan films on electrodes

Perform electrochemical deposition of chitosan polysaccharide under bias to generate alternative biocompatible film substrates.

▶ 06:29
4
Measure electrochemical transduction in functionalized chitosan

Apply electrochemical stimuli to chitosan films and measure electrical responses to validate transduction capability.

▶ 07:51
5
Functionalize films with proteins via enzymatic assembly

Immobilize proteins onto polysaccharide films using enzymatic cross-linking or assembly methods to create bioactive surfaces.

▶ 09:58
6
Integrate biofunctionalized surfaces into lab-on-chip platform

Assemble electrodeposited and functionalized films into microfluidic or lab-on-chip devices for interactive bioanalysis applications.

▶ 11:32
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