Home › Cell Biology › Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue
Cell Biology JoVE (Open Access) Citable · DOI

Cerenkov Luminescence Imaging of Interscapular Brown Adipose Tissue

DOI: 10.3791/51790-v
What you'll learn
  • ✓ Perform Cerenkov Luminescence Imaging (CLI) of brown adipose tissue using 18F-FDG in mice
  • ✓ Interpret CLI signals under activated and depressed metabolic conditions
  • ✓ Apply spectral unmixing and multispectral tomography to BAT imaging studies
Protocol

In this video report, we show the application of Cerenkov Luminescence Imaging (CLI) for interscapular brown adipose tissue in mice under activated and depressed conditions.

Difficulty
advanced
Total time
~2–3 hrs per mouse (including radiotracer synthesis, animal preparation, imaging acquisition, and post-processing)
Model organism
Mouse (strain not specified)
Biosafety
BSL-1

Steps

1
Perform in vivo Cerenkov Luminescence Imaging with 18F-FDG

Inject mice intravenously with 18F-fluorodeoxyglucose (18F-FDG) and acquire Cerenkov Luminescence images of interscapular brown adipose tissue using appropriate optical imaging hardware. Image acquisition occurs under activated and depressed metabolic conditions.

▶ 02:24
2
Apply spectral unmixing and multispectral tomography analysis

Process acquired CLI images using spectral unmixing techniques and perform multispectral Cerenkov Luminescence Tomography to enhance signal specificity and three-dimensional localization of brown adipose tissue activity.

▶ 02:39
3
Evaluate representative Cerenkov Luminescence imaging results

Analyze and interpret representative CLI images demonstrating brown adipose tissue signal under different metabolic states, comparing activated versus depressed conditions to assess protocol efficacy.

▶ 04:30
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