Typically, the mouse neck injection model is used for evaluate pruritogen-induced scratch behaviors. However, the model provides information only on itch, not pain. Here, a cheek injection model is introduced in mice which can be used to simultaneously measure pain and itch-related behaviors.
Total time
~45 min per mouse (including injection, observation, and behavior quantification)
Model organism
Mouse (strain not specified)
Steps
1
Introduce cheek injection model methodology
Overview of the cheek injection technique as an alternative to neck injection, explaining the anatomical and procedural basis for simultaneous pain and itch measurement in mice.
▶ 00:53
2
Compare neck injection model approach
Review the traditional neck injection model for itch-only assessment, highlighting its limitations and the rationale for developing the cheek injection alternative.
▶ 02:36
3
Analyze pain and itch behavior results
Interpret representative data from behavioral analyses demonstrating pain-related responses and itch-related scratch behaviors measured simultaneously using the cheek injection model.
▶ 03:12
4
Summarize model applications and advantages
Synthesize conclusions regarding the cheek injection model's utility for dual-parameter behavioral measurement in pruritogen research and future experimental design considerations.
▶ 04:08