Home › Cell Biology › Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings
Cell Biology JoVE (Open Access) Citable · DOI

Combined Recording of Mechanically Stimulated Afferent Output and Nerve Terminal Labelling in Mouse Hair Follicle Lanceolate Endings

DOI: 10.3791/53854-v
What you'll learn
  • ✓ Dissect mouse ear skin for afferent recording
  • ✓ Set up electrophysiology rig for mechanostimulation
  • ✓ Record stimulus-evoked action potentials from lanceolate endings
  • ✓ Identify nerve terminals via labelling after recording
Protocol

Biopharma Insights A simple and novel technique for recording afferent discharge due to mechanical stimulation of lanceolate terminals of palisade endings innervating mouse ear skin hair follicles is presented.

Difficulty
advanced
Total time
~2-3 hours per mouse for preparation and recording
Model organism
Mouse
Biosafety
BSL-1

Steps

1
Prepare ears for electrophysiology

Harvest and dissect mouse ear skin to expose hair follicles for recording.

▶ 01:13
2
Set up electrophysiology recording rig

Position electrodes and mechanical stimulator to target lanceolate endings.

▶ 04:25
3
Record stimulus-evoked action potentials

Apply mechanical stimuli and record afferent discharge from nerve terminals.

▶ 08:17
4
Analyze representative results from lanceolate endings

Review sample recordings demonstrating successful afferent response and labelling.

▶ 10:13
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