Neuroscience JoVE (Open Access) Citable · DOI

Concurrent Recording of Co-localized Electroencephalography and Local Field Potential in Rodent

Name: Concurrent Recording of Co-localized Electroencephalography and Local Field Potential in Rodent
Uploaded: 2026-06-17
Duration: 8 min 31 s
Description: Although electroencephalography (EEG) is widely used as a non-invasive technique for recording neural activities of the brain, our understanding of the ...

DOI: 10.3791/56447-v

What you'll learn

✓ Perform concurrent EEG and multi-laminar LFP recording in anesthetized rodents
✓ Execute skull burr hole drilling with minimal EEG signal distortion
✓ Set up co-localized microelectrode placement for simultaneous neural recordings
✓ Interpret combined EEG and LFP data from single brain region

Protocol

This protocol describes a simple method for concurrent recording of co-localized electroencephalography (EEG) and multi-laminar local field potential in an anesthetized rat. A burr hole drilled in the skull for the insertion of a microelectrode is shown to produce negligible distortion of the EEG signal.

Difficulty

advanced

Total time

~3-4 hours per rat (including anesthesia induction, surgery, electrode placement, and stabilization)

Model organism

Rat (anesthetized)

Biosafety

BSL-1

Steps

Perform surgical skull burr hole drilling

Drill a burr hole in the skull under anesthesia for microelectrode insertion. This procedure produces negligible distortion of concurrent EEG recordings.

▶ 00:23

Configure co-localized EEG and LFP setup

Position microelectrode array for multi-laminar local field potential recording while maintaining surface EEG electrodes at the same brain region to ensure spatial co-localization.

▶ 03:47

Acquire and validate concurrent recordings

Collect simultaneous EEG and multi-laminar LFP signals and verify data quality, signal alignment, and absence of cross-talk between recording modalities.

▶ 06:41

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