This paper describes the assembly process and operation of a bench-scale photosynthetic bioreactor that can be used, in conjunction with other methods, to estimate pertinent kinetic growth parameters. This system continuously monitors the pH, light, and temperature using sensors, a data acquisition and control unit, and open-source data acquisition software.
Total time
~1–2 days (setup and calibration ~2–3 hours; kinetic growth monitoring 24+ hours depending on culture phase)
Model organism
Algae (species not specified)
Steps
1
Set up and configure pH electrode system
Install the pH electrode probe into the bioreactor vessel and connect it to the data acquisition and control unit, ensuring proper electrical and mechanical connections.
▶ 01:04
2
Calibrate pH probe using buffer standards
Perform two-point or multi-point pH calibration using standard buffer solutions to establish the probe's measurement accuracy before experimental use.
▶ 03:27
3
Configure bioreactor for algal growth experiment
Set up the complete photosynthetic bioreactor including light source, temperature control, and pH sensor integration; configure open-source data acquisition software for parameter logging.
▶ 04:22
4
Monitor logged data and maintain reactor controls
Continuously acquire and verify pH, light intensity, and temperature data throughout the kinetic growth experiment; adjust control parameters as needed to maintain experimental conditions.
▶ 07:10