Home › Neuroscience › Detecting Pre-Stimulus Source-Level Effects on Object Perception with Magnetoencephalography
Neuroscience JoVE (Open Access) Citable · DOI

Detecting Pre-Stimulus Source-Level Effects on Object Perception with Magnetoencephalography

DOI: 10.3791/60120-v
What you'll learn
  • ✓ Set up MEG recording equipment and participant preparation protocols
  • ✓ Monitor real-time MEG signal quality during stimulus presentation
  • ✓ Analyze pre-stimulus source-level connectivity between brain regions
  • ✓ Interpret pre-stimulus neural effects on object perception outcomes
Protocol

Biopharma Insights This article describes how to set up an experiment that allows detecting pre-stimulus source-level influences on object perception using magnetoencephalography (MEG). It covers stimulus material, experimental design, MEG recording, and data analysis.

Difficulty
advanced
Total time
~2–3 hours per participant (including setup, recording session, and real-time monitoring)

Steps

1
Prepare participant and MEG equipment setup

Position participant in MEG scanner, apply fiducial markers, and configure magnetometer sensors. Establish baseline signal quality and environmental shielding parameters.

▶ 00:34
2
Initiate MEG recording and monitor signal quality

Begin stimulus presentation protocol, continuously monitor real-time MEG waveforms for artifact and signal integrity, and maintain participant comfort and attention throughout acquisition.

▶ 04:19
3
Analyze pre-stimulus connectivity between regions

Apply source-level analysis and connectivity metrics to pre-stimulus MEG data, identifying coherence or coupling between anatomically defined regions of interest prior to stimulus onset.

▶ 06:21
4
Interpret pre-stimulus source-level effects results

Examine statistical outcomes of pre-stimulus neural activity and its correlation with subsequent object perception performance, and contextualize findings within predictive coding frameworks.

▶ 07:44
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