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Neuroscience JoVE (Open Access) Citable · DOI

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

DOI: 10.3791/1543-v
What you'll learn
  • ✓ Perform stereomicroscope-guided dissection of mouse dentate gyrus
  • ✓ Isolate dentate gyrus tissue from adult mouse brain
  • ✓ Apply proper dissection technique for hippocampal subregion extraction
Protocol

Biopharma Insights A dissection technique for removal of the dentate gyrus from adult mouse under a stereomicroscope was demonstrated in this video-recorded protocol.

Difficulty
advanced
Total time
~20–30 min/mouse
Model organism
Mouse (adult, strain unspecified)
Biosafety
BSL-1

Steps

1
Understand dentate gyrus anatomy and dissection rationale

Introduction covers the anatomical location of the dentate gyrus within the hippocampus and the rationale for isolating this subregion for downstream applications.

▶ 00:15
2
Perform stereomicroscope-guided dentate gyrus dissection

Step-by-step protocol for removing the dentate gyrus from the adult mouse brain under stereomicroscopic visualization, including positioning, orientation, and tissue removal technique.

▶ 02:56
3
Assess dissection success and tissue integrity

Representative results demonstrate the expected appearance and quality of successfully isolated dentate gyrus tissue.

▶ 06:38
4
Review dissection outcomes and practical considerations

Conclusion summarizes key technical points and applications of the isolated dentate gyrus for further analysis.

▶ 07:03
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