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Analytical Chem JoVE (Open Access) Citable · DOI

Dissection of Human Vitreous Body Elements for Proteomic Analysis

DOI: 10.3791/2455-v
What you'll learn
  • ✓ Dissect and isolate vitreous body structures from post mortem human eyes
  • ✓ Differentiate anterior segment, vitreous core, base, and cortex regions
  • ✓ Prepare vitreous samples for downstream proteomic analysis
Protocol

This video shows an effective technique for differentiating and dissecting the various semi-transparent structures of the human vitreous body in post mortem eyes.

Difficulty
advanced
Total time
~45 min per eye
Biosafety
BSL-1

Steps

1
Dissect anterior segment from post mortem eye

Remove and separate the anterior structures of the post mortem eye to expose the vitreous body. This preparatory step allows access to internal vitreous compartments.

▶ 00:22
2
Aspirate vitreous core sample

Extract the central vitreous core using aspiration technique. This isolates the gel-like core region for proteomic analysis.

▶ 01:02
3
Open eye using butterfly dissection method

Carefully butterfly or open the eye to expose remaining vitreous structures and allow access to peripheral regions for further dissection.

▶ 02:21
4
Dissect vitreous base from eye wall

Isolate and remove the vitreous base, the region where vitreous gel attaches to the retina and ciliary body. This structure requires careful separation from surrounding tissues.

▶ 02:53
5
Dissect vitreous cortex layer

Extract the cortical layer of the vitreous, the peripheral gel region distinct from the core. This completes anatomical compartmentalization for proteomic analysis.

▶ 04:12
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