Home Cell Biology Dissection of Organs from the Adult Zebrafish
Cell Biology JoVE (Open Access) Citable · DOI

Dissection of Organs from the Adult Zebrafish

DOI: 10.3791/1717-v
What you'll learn
  • Identify and locate major organs in adult zebrafish anatomy
  • Execute precise dissection techniques for zebrafish organ extraction
  • Distinguish between male and female reproductive organs in zebrafish
Protocol

Biopharma Insights This protocol describes a procedure for identifying and dissecting organs from the adult zebrafish.

Difficulty
intermediate
Total time
~45 minutes per fish
Model organism
Danio rerio (adult zebrafish)
Biosafety
BSL-1

Steps

1
Understand zebrafish organ dissection overview

Review the protocol objectives and experimental context for adult zebrafish organ identification and extraction procedures.

▶ 00:51
2
Dissect testes, gastrointestinal, and kidney systems

Perform surgical dissection to expose and isolate the testes, gastrointestinal tract, swim bladder, and kidney from adult zebrafish specimens.

▶ 01:05
3
Dissect ovary, heart, and brain tissues

Execute careful dissection to extract the ovary, heart, and brain from adult zebrafish while preserving tissue integrity.

▶ 05:45
4
Review protocol outcomes and applications

Summarize key findings and discuss the practical applications of zebrafish organ dissection in research contexts.

▶ 09:50
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