Home › Cell Biology › Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System
Cell Biology JoVE (Open Access) Citable · DOI

Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System

DOI: 10.3791/55454-v
What you'll learn
  • ✓ Operate a nose-only aerosol inhalation exposure system for rodents
  • ✓ Generate and characterize dry powder and nebulized drug aerosols
  • ✓ Measure lung deposition and assess inhaled drug efficacy/safety in mice
  • ✓ Design pre-clinical inhalation studies for drug development
Protocol

The inhalation unit described herein can generate, sample for characterization, and uniformly deposit a drug aerosol in the lungs of rodents. This enables the pre-clinical determination of the efficacy and safety of drug doses deposited in the lungs; key data enabling clinical inhaled drug development.

Difficulty
advanced
Total time
~1–2 hours per exposure session; multi-day study protocol dependent on experimental design
Model organism
Mouse (unspecified strain)
Biosafety
BSL-1

Steps

1
Generate dry powder and nebulized aerosols

Prepare and operate aerosol generation apparatus to produce pharmaceutical dry powder or nebulized liquid aerosol for inhalation delivery. Understand equipment configuration and principles of aerosol generation.

▶ 00:57
2
Assemble exposure system and deliver aerosol

Configure the nose-only inhalation chamber, position the mouse, and deliver the generated aerosol to the respiratory tract while monitoring exposure parameters in real time.

▶ 02:46
3
Quantify real-time aerosol delivery and deposition

Measure and analyze aerosol characterization data (particle size, concentration, lung deposition) during and after exposure to confirm uniform drug distribution in target tissue.

▶ 05:28
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