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Cell Biology JoVE (Open Access) Citable · DOI

Dual-mode Imaging of Cutaneous Tissue Oxygenation and Vascular Function

DOI: 10.3791/2095-v
What you'll learn
  • ✓ Operate a dual-mode imaging system for non-contact cutaneous assessment
  • ✓ Generate and interpret tissue oxygenation maps using multispectral imaging
  • ✓ Acquire vascular function data via dynamic thermographic imaging
  • ✓ Co-register oxygenation and vascular maps for integrated analysis
Protocol

A dual-mode imaging system was developed for non-contact assessment of cutaneous tissue oxygenation and vascular function.

Difficulty
advanced
Total time
~45 min–1 hr per subject

Steps

1
Understand dual-mode imaging system principles

Review the theoretical basis and motivations for simultaneous multispectral and thermographic imaging of cutaneous tissue. Understand how the system integrates two complementary optical modes.

▶ 01:06
2
Map tissue oxygenation by multispectral imaging

Acquire multispectral image data across relevant wavelengths to quantify tissue oxygen saturation and perfusion in cutaneous regions. Process and display oxygenation maps.

▶ 02:38
3
Map vascular function by dynamic thermographic imaging

Use thermal imaging to assess dynamic vascular responses and perfusion changes in real-time. Generate thermal maps of cutaneous vascular activity.

▶ 05:26
4
Co-register tissue oxygenation and vascular maps

Align and fuse multispectral oxygenation maps with thermographic vascular maps to create integrated spatial datasets. Validate registration accuracy.

▶ 07:26
5
Analyze representative results and data interpretation

Review example outputs demonstrating merged oxygenation and vascular maps. Interpret spatial correlations and clinical relevance of dual-mode assessment.

▶ 09:03
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