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Pharmacology JoVE (Open Access) Citable · DOI

Early Detection of Drug-Induced Renal Hemodynamic Dysfunction Using Sonographic Technology in Rats

DOI: 10.3791/52409-v
What you'll learn
  • ✓ Perform renal sonographic imaging in a drug-induced nephrotoxicity rat model
  • ✓ Identify hemodynamic dysfunction markers using ultrasound technology
  • ✓ Correlate sonographic findings with creatinine and histological kidney injury
Protocol

Early stage hemodynamic dysfunction is critical to the development of kidney disease. Yet, detection methodologies are limited. Recent advances in sonography provide a noninvasive, accurate option for early detection of kidney injury. This study outlines a step-by-step, sonographic methodology for detecting kidney dysfunction using a drug-induced nephrotoxicity rat model.

Difficulty
advanced
Total time
~3-4 days (including cisplatin treatment, imaging, and endpoint collection)
Model organism
Rat (nephrotoxicity model)
Biosafety
BSL-1

Steps

1
Perform transverse sonographic imaging of rat kidney

Acquire ultrasound images of the kidney in transverse orientation using sonographic technology. This step establishes baseline imaging technique for hemodynamic assessment.

▶ 01:28
2
Induce nephrotoxicity using cisplatin treatment

Administer cisplatin to rats to generate drug-induced renal dysfunction. This creates the experimental model for observing early hemodynamic changes.

▶ 03:56
3
Assess creatinine, histology and ultrasound outcomes

Collect and analyze creatinine levels, perform histological examination, and capture ultrasound images post-cisplatin treatment to detect renal hemodynamic dysfunction.

▶ 04:31
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