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Biochemistry JoVE (Open Access) Citable · DOI

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Name: Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio
Uploaded: 2026-10-13
Duration: 11 min 27 s
Description: Learn CRISPR/Cas9 genome editing in zebrafish to generate gene knockouts using microinjection and heteroduplex mobility assay. This robust protocol enables researchers at all levels to study gene function in Danio rerio.

DOI: 10.3791/56969-v

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