Home › Cell Biology › Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)
Cell Biology JoVE (Open Access) Citable · DOI

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

DOI: 10.3791/55587-v
What you'll learn
  • ✓ Dissociate and plate hPSC-derived cardiomyocytes on multi-electrode arrays
  • ✓ Measure and record field potential signals from cultured cardiac tissue
  • ✓ Analyze QT and RR intervals from electrophysiological recordings
  • ✓ Assess signal quality and troubleshoot MEA-based cardiac electrophysiology
Protocol

Electrophysiological characterization of cardiomyocytes derived from human Pluripotent Stem Cells (hPSC-CMs) is crucial for cardiac disease modeling and for determining drug responses. This protocol provides the necessary information to dissociate and plate hPSC-CMs on multi-electrode arrays, measure their field potential, and a method for analyzing QT and RR intervals.

Difficulty
advanced
Total time
~3–5 days (including hPSC-CM differentiation and maturation on MEA; protocol shown focuses on MEA setup and recording, ~2–3 hours per sample)
Model organism
Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs)
Biosafety
BSL-1

Steps

1
Coat MEA chips with extracellular matrix

Apply poly(dimethylsiloxane) or fibronectin coating to multi-electrode array chips to promote cell adhesion and attachment of hPSC-CMs.

▶ 01:13
2
Dissociate hPSC-CMs and plate onto arrays

Enzymatically dissociate hPSC-derived cardiomyocytes and seed them onto coated MEA chips at optimal density for electrophysiological recording.

▶ 03:07
3
Remove ring and refresh culture medium

Remove isolation ring from MEA chip and perform medium exchange to maintain cell viability and optimal recording conditions.

▶ 05:23
4
Verify signal quality and initiate recording

Check baseline electrophysiological signal quality on MEA electrodes and configure acquisition parameters before commencing field potential measurements.

▶ 06:17
5
Acquire and analyze MEA field potential recordings

Record spontaneous electrical activity from hPSC-CMs and extract QT and RR interval data for cardiac electrophysiological characterization.

▶ 08:47
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