Home › Cell Biology › Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis
Cell Biology JoVE (Open Access) Citable · DOI

Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis

DOI: 10.3791/52940-v
What you'll learn
  • ✓ Perform a reproducible femoral critical-size defect in mice using plate osteosynthesis.
  • ✓ Stabilize segmental bone defects with locking plate and four-screw fixation.
  • ✓ Assess surgical technique for low-morbidity bone tissue engineering models.
  • ✓ Evaluate femoral bone reconstruction outcomes post-operatively.
Protocol

Although mouse models are invaluable tools for bone tissue engineering, models of long bone defects are sparse. This need motivated development of the present protocol which uses a locking plate with four screws and a dedicated jig to perform and stabilize a reproducible, femoral, critical-size defect with low morbidity.

Difficulty
advanced
Total time
~90–120 min per mouse (surgical procedure + stabilization + recovery monitoring)
Model organism
Mouse (strain not specified)
Biosafety
BSL-1

Steps

1
Create femoral segmental critical-size defect

Establish a reproducible segmental bone defect in the mouse femur using standardized surgical technique and dedicated jig to ensure consistent defect geometry and location.

▶ 00:48
2
Stabilize defect with locking plate osteosynthesis

Apply a locking plate with four screws to stabilize the femoral defect, ensuring reproducible fixation and low morbidity during the healing period.

▶ 03:35
3
Assess bone reconstruction and surgical outcomes

Monitor and evaluate representative results of mouse femoral bone reconstruction following osteosynthesis-facilitated healing to validate defect model efficacy.

▶ 04:59
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