Biopharma Insights Understanding how cells are modulated by exposure to shock waves can help identify the mechanisms behind injuries triggered from blast events. This protocol uses custom-built shock tube equipment to apply shock waves at a range of pressures to cell monolayers and to identify the subsequent effects on cell viability.
Difficulty
advanced
Total time
~2 days per experiment
Model organism
Cell monolayers
Biosafety
BSL-1
Steps
1
Prepare and culture cell monolayers
Culture cells to the desired density and prepare samples for blast exposure.
▶ 01:00
2
Assemble the shock tube equipment
Set up the custom-built shock tube apparatus according to the protocol.
▶ 02:27
3
Operate the shock tube for blast exposure
Generate and deliver shock waves at specified pressures to the cell samples.
▶ 04:46
4
Perform cell viability assay
Measure cell viability after blast exposure to evaluate its effects.
▶ 07:01
5
Analyze blast effects on cell growth
Interpret viability results to determine the impact of different pressure levels.
▶ 08:52
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