Home › Microscopy & Imaging › Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
Microscopy & Imaging JoVE (Open Access) Citable · DOI

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

DOI: 10.3791/59822-v
What you'll learn
  • ✓ Prepare fission yeast cultures and samples for live-cell microscopy
  • ✓ Perform fluorescence live-cell imaging of mitotic and meiotic nuclear dynamics
  • ✓ Analyze time-lapse images to track protein behavior and nuclear structures
Protocol

Here, we present live-cell imaging which is a non-toxic microscopy method that allows researchers to study protein behavior and nuclear dynamics in living fission yeast cells during mitosis and meiosis.

Difficulty
intermediate
Total time
~3-4 hours per experiment (culture preparation + imaging + analysis)
Model organism
Schizosaccharomyces pombe (fission yeast)
Biosafety
BSL-1

Steps

1
Culture and prepare fission yeast samples

Grow fission yeast cultures under appropriate conditions and prepare samples for microscopy by mounting cells on slides or in imaging chambers.

▶ 01:06
2
Perform live-cell fluorescence microscopy imaging

Set up and execute live-cell imaging on prepared fission yeast samples using fluorescence microscopy to capture time-lapse sequences of mitotic and meiotic nuclear dynamics.

▶ 07:12
3
Analyze time-lapse images and nuclear dynamics

Process and analyze acquired microscopy images using image analysis software to track nuclear structures, protein localization, and dynamic events during cell division.

▶ 08:09
4
Interpret results of mitotic and meiotic events

Review and characterize observed nuclear dynamics patterns in both mitotic and meiotic cells to validate experimental observations.

▶ 10:05
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