Biopharma Insights We present a method for inducing elevated intraocular pressure (IOP), by injecting magnetic microspheres into the rat eye, to model glaucoma. This leads to strong pressure rises, and extensive neuronal death. This protocol is easy to perform, does not require repeat injections, and produces stable long-lasting IOP rises.
Total time
~45 min per rat (injection + initial IOP measurement); long-term monitoring over weeks
Model organism
Rat (strain not specified)
Steps
1
Inject magnetic microspheres into rat eye
Perform ocular injection of magnetic microspheres into the anterior chamber of the rat eye to induce elevated intraocular pressure. The method produces stable, long-lasting IOP elevation without requiring repeat injections.
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2
Measure intraocular pressure and assess outcomes
Quantify induced intraocular pressure elevation and evaluate resulting neuronal death in the glaucoma model. Document pressure stability and extent of neuronal damage to validate the experimental model.
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