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Biochemistry JoVE (Open Access) Citable · DOI

Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins

Name: Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
Uploaded: 2026-10-12
Duration: 10 min 21 s
Description: Learn expression and purification of FAH domain-containing proteins using E. coli and nickel NTA affinity chromatography. This protocol enables crystallization and enzyme assays for studying metabolic regulation.

DOI: 10.3791/59729-v

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