Home › Microbiology › Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus
Microbiology JoVE (Open Access) Citable · DOI

Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus

DOI: 10.3791/55508-v
What you'll learn
  • ✓ Isolate and culture fat body tissue from Aedes aegypti mosquitoes
  • ✓ Maintain live organ tissue in vitro for functional studies
  • ✓ Assess fat body metabolic responses to experimental stimuli
  • ✓ Apply insect organ culture techniques to vector biology research
Protocol

Biopharma Insights The fat body is the central metabolic organ in insects. We present a live organ culture system that enables the user to study the responses of isolated fat body tissue to various stimuli.

Difficulty
intermediate
Total time
~2–3 hours per culture setup (tissue isolation to culture establishment)
Model organism
Aedes aegypti (mosquito)
Biosafety
BSL-1

Steps

1
Gather materials and equipment for organ culture

Assemble all required tools, culture media, dissection instruments, and mosquito specimens needed to establish the fat body organ culture system.

▶ 01:43
2
Dissect and isolate fat body tissue from mosquitoes

Perform sterile dissection of Aedes aegypti to extract the fat body organ and prepare it for live culture conditions.

▶ 03:04
3
Culture and maintain isolated fat body in vitro

Place dissected fat body tissue in culture medium and establish conditions for sustained viability and metabolic activity during experimental observation.

▶ 04:59
4
Evaluate fat body responses to experimental stimuli

Measure and document metabolic or physiological responses of cultured fat body tissue to applied treatments or environmental conditions.

▶ 05:56
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