Home › Analytical Chem › Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
Analytical Chem JoVE (Open Access) Citable · DOI

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

DOI: 10.3791/58757-v
What you'll learn
  • ✓ Perform Flash NanoPrecipitation using confined impinging jet mixer for hydrophobic encapsulation
  • ✓ Encapsulate hydrophilic compounds in inverted nanoparticles using multi-inlet vortex mixer
  • ✓ Scale polymeric nanoparticle formulations from lab to larger production volumes
  • ✓ Characterize core-shell nanoparticle properties and encapsulation efficiency
Protocol

Flash NanoPrecipitation (FNP) is a scalable approach to produce polymeric core-shell nanoparticles. Lab-scale formulations for the encapsulation of hydrophobic or hydrophilic therapeutics are described.

Difficulty
intermediate
Total time
~2-4 hours per formulation (including mixing, precipitation, and initial characterization)

Steps

1
Encapsulate hydrophobic compounds using confined impinging jet mixer

Prepare and load hydrophobic therapeutic compounds into polymeric core-shell nanoparticles using the CIJ mixer apparatus. This method enables rapid, scalable precipitation for hydrophobic drug encapsulation.

▶ 00:51
2
Encapsulate hydrophilic ovalbumin in inverted nanoparticles

Formulate inverted nanoparticles with hydrophilic protein payloads using the small-scale multi-inlet vortex mixer. This approach inverts the core-shell architecture for water-soluble therapeutics.

▶ 04:08
3
Modify formulation protocol for larger-scale production

Adapt laboratory-scale Flash NanoPrecipitation procedures for increased batch sizes and production volumes while maintaining nanoparticle quality and encapsulation efficiency.

▶ 06:44
4
Characterize polymeric nanoparticle properties and composition

Analyze resulting nanoparticles for size distribution, morphology, core composition, and encapsulation efficiency using appropriate analytical techniques. Compare hydrophobic and hydrophilic formulation outcomes.

▶ 07:33
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