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Analytical Chem JoVE (Open Access) Citable · DOI

Generation of Electronic Cigarette Aerosol by a Third-Generation Machine-Vaping Device: Application to Toxicological Studies

DOI: 10.3791/58095-v
What you'll learn
  • ✓ Assemble and operate a third-generation e-cig aerosol generation device
  • ✓ Generate standardized, reproducible e-cig aerosol for animal inhalation exposure
  • ✓ Analyze physicochemical properties of generated e-cig aerosol
  • ✓ Perform equipment maintenance and cleaning protocols post-exposure
Protocol

Electronic cigarette (e-cig) users are increasing worldwide. Little, however, is known about the health effects induced by inhaled e-cig aerosols. This article describes an e-cig aerosol generation technique suitable for animal exposures and subsequent toxicological studies. Such protocols are required to establish experimentally reproducible and standardized e-cig exposure systems.

Difficulty
intermediate
Total time
~2–4 hours per exposure session (setup, generation, analysis, cleaning)
Biosafety
BSL-1

Steps

1
Prepare e-cig device and exposure apparatus

Assemble the third-generation e-cig machine-vaping device, configure airflow settings, and prepare the exposure chamber for standardized aerosol generation.

▶ 00:35
2
Generate aerosol and conduct animal exposure

Operate the machine-vaping device to generate e-cig aerosol under controlled puffing parameters, direct aerosol into the exposure chamber, and expose subjects for the designated duration.

▶ 02:11
3
Collect and analyze aerosol samples post-exposure

Retrieve aerosol samples from the exposure chamber and measure physicochemical properties such as particle size distribution, concentration, and composition.

▶ 05:14
4
Clean and maintain the vaping device

Disassemble components, rinse aerosol-contact surfaces, and perform routine maintenance to ensure device functionality and reproducibility for subsequent exposures.

▶ 07:18
5
Interpret aerosol generation results and quality control

Review analytical data from aerosol characterization, validate reproducibility against exposure parameters, and confirm suitability for toxicological investigation.

▶ 07:50
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