Home › Cell Biology › High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources
Cell Biology JoVE (Open Access) Citable · DOI

High-density Electroencephalographic Acquisition in a Rodent Model Using Low-cost and Open-source Resources

DOI: 10.3791/54908-v
What you'll learn
  • ✓ Construct a low-cost high-density EEG headpiece for rodents
  • ✓ Perform surgical implantation of chronic transcranial EEG electrodes
  • ✓ Record, extract, and process multi-channel EEG signals from mice
Protocol

Instructions for the low-cost construction and surgical implantation of a chronic transcranial high-density electroencephalographic montage into mice are provided. Signal recording, extraction, and processing techniques are also described.

Difficulty
advanced
Total time
~2-3 hours per mouse (surgery); ~1 week post-operative recovery before recording
Model organism
Mouse

Steps

1
Design and construct headpiece assembly

Build a custom high-density EEG headpiece using open-source resources and low-cost materials. This section covers the mechanical design and fabrication of the electrode mounting structure.

▶ 00:55
2
Construct adapter and map recording channels

Assemble the connector adapter and establish channel mapping for multi-electrode recording. This ensures proper signal routing from implanted electrodes to recording equipment.

▶ 04:22
3
Perform surgical implantation of headpiece

Execute the surgical procedure for chronic transcranial implantation of the EEG headpiece into the mouse skull. This is a sterile procedure requiring anesthesia and IACUC approval.

▶ 06:39
4
Setup signal extraction and begin recording

Configure the recording system and begin acquiring EEG signals from the implanted electrodes. Connect the headpiece to amplification and digitization hardware.

▶ 08:59
5
Process and analyze recorded EEG data

Apply signal processing techniques and demonstrate applications of the acquired high-density EEG recordings. Results showcase the utility of this low-cost approach.

▶ 10:16
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