Home › Cell Biology › High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
Cell Biology JoVE (Open Access) Citable · DOI

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart

DOI: 10.3791/1781-v
What you'll learn
  • ✓ Perform left anterior descending artery ischemia-reperfusion surgery in mice
  • ✓ Acquire high-frequency echocardiographic M-mode and Doppler imaging data
  • ✓ Measure myocardial strain using VevoStrain speckle tracking algorithm
  • ✓ Quantify contractility and mitral regurgitation in ischemia-reperfused hearts
Protocol

High frequency Doppler ultrasound is a novel technology for assessing regional myocardial function. This work presents first evidence demonstrating applicability of this versatile imaging platform for the repeated measure of myocardial strain, dp/dt, and mitral regurgitation in the ischemia-reperfused (IR) murine heart.

Difficulty
advanced
Total time
~2–3 hours per mouse (surgery ~45–60 min, imaging ~60–90 min)
Model organism
Mouse (strain not specified)
Biosafety
BSL-1

Steps

1
Understand high-frequency ultrasound imaging principles

Review the theoretical basis and advantages of high-frequency Doppler ultrasound for assessing regional myocardial function in murine cardiac models.

▶ 00:47
2
Perform left anterior descending artery ischemia-reperfusion

Conduct surgical procedure to induce temporary LAD occlusion and controlled reperfusion in the mouse heart under anesthesia.

▶ 01:52
3
Acquire M-mode echocardiographic images

Position the mouse and obtain standard M-mode ultrasound images of the left ventricle using high-frequency transducers.

▶ 04:55
4
Apply VevoStrain speckle tracking algorithm

Use speckle tracking software to quantify myocardial strain and contractility (dp/dt) from acquired echocardiographic data.

▶ 06:10
5
Measure Doppler flow and mitral regurgitation

Perform pulsed and continuous-wave Doppler imaging to assess blood flow velocities and quantify mitral regurgitation severity.

▶ 07:29
6
Interpret and analyze representative imaging results

Review typical M-mode, strain, and Doppler outputs from ischemia-reperfused and control hearts to validate protocol efficacy.

▶ 09:15
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