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Immunology JoVE (Open Access) Citable · DOI

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

DOI: 10.3791/52718-v
What you'll learn
  • ✓ Acquire and optimize high-frequency ultrasound B-mode images of mouse cervical lymph nodes
  • ✓ Perform image-guided biopsy and tissue collection from cervical lymph nodes
  • ✓ Quantify lymph node morphology, volume, and blood flow from ultrasound images
  • ✓ Process lymph node tissue for histological evaluation and analysis
Protocol

This protocol describes the application of high-frequency ultrasound (HFUS) for imaging mouse cervical lymph nodes. This technique optimizes visualization and quantification of cervical lymph node morphology, volume and blood flow. Image-guided biopsy of cervical lymph nodes and processing of lymph tissue for histological evaluation is also demonstrated.

Difficulty
advanced
Total time
~45–60 min per mouse (imaging, biopsy, and tissue processing)
Model organism
Mouse (unspecified strain)
Biosafety
BSL-1

Steps

1
Prepare mouse and ultrasound imaging system

Set up equipment, anesthesia, and positioning for high-frequency ultrasound imaging of cervical lymph nodes.

▶ 01:42
2
Acquire B-mode ultrasound images of cervical lymph nodes

Optimize probe positioning and scanning parameters to visualize and capture high-frequency ultrasound images of cervical lymph node anatomy and morphology.

▶ 02:41
3
Perform image-guided biopsy of cervical lymph nodes

Use ultrasound guidance to locate and obtain tissue samples from cervical lymph nodes via needle biopsy.

▶ 04:41
4
Analyze lymph node volume and blood flow from images

Process acquired ultrasound images to quantify lymph node volume, morphometry, and blood flow characteristics using image analysis software.

▶ 06:51
5
Review representative imaging and biopsy results

Examine example high-frequency ultrasound images, biopsy outcomes, and processed data illustrating successful protocol application.

▶ 08:20
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