Home›Cell Biology›High Pressure Single Crystal Diffraction at PX^2
Cell BiologyJoVE (Open Access)Citable · DOI
High Pressure Single Crystal Diffraction at PX^2
DOI: 10.3791/54660-v
What you'll learn
✓Set up and optimize single crystal X-ray diffraction in a diamond anvil cell
✓Collect high-pressure diffraction data using synchrotron radiation
✓Process and analyze diffraction data using ATREX and RSV software
Protocol
In this report, we describe detailed procedures for carrying out single crystal X-ray diffraction experiments with a diamond anvil cell at the GSECARS 13-BM-C beamline at the Advanced Photon Source. ATREX and RSV programs are used to analyze the data.
Difficulty
advanced
Total time
~4–6 hours per sample (including setup, data collection, and preliminary analysis)
Steps
1
Collect single crystal diffraction data at beamline
Position the diamond anvil cell and sample at the GSECARS 13-BM-C beamline, align optics, and acquire X-ray diffraction images under controlled pressure conditions.
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2
Analyze diffraction data with ATREX and RSV
Process raw diffraction images and extract unit cell parameters and structural information using ATREX and RSV data reduction programs.