Home › Cell Biology › High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots
Cell Biology JoVE (Open Access) Citable · DOI

High-throughput, Automated Extraction of DNA and RNA from Clinical Samples using TruTip Technology on Common Liquid Handling Robots

DOI: 10.3791/50356-v
What you'll learn
  • ✓ Set up automated nucleic acid extraction on liquid handling robots
  • ✓ Execute high-throughput DNA/RNA extraction from clinical samples using TruTip technology
  • ✓ Evaluate extraction speed, fidelity, and purity outcomes for clinical applications
Protocol

TruTip is a simple nucleic acid extraction technology whereby a porous, monolithic binding matrix is inserted into a pipette tip. Consequently, the sample preparation format is compatible with most liquid handling instruments, and can be used for many medium to high-throughput clinical applications and sample types.

Difficulty
intermediate
Total time
~45 min to 1.5 hrs per 96-well plate (depending on sample type and instrument throughput)
Biosafety
BSL-2

Steps

1
Familiarize with Hamilton STAR liquid-handling robot

Review the Hamilton STAR robotic platform capabilities and integration with TruTip extraction technology for compatible sample preparation workflows.

▶ 02:15
2
Set up 96-well DNA extraction from whole blood

Configure the liquid handling robot and prepare a 96-well plate setup for parallel DNA extraction from clinical whole blood samples using TruTip pipette tips.

▶ 02:51
3
Perform sample lysis and reagent distribution

Execute automated lysis of blood samples and distribute extraction reagents across the 96-well format using the liquid handling robot.

▶ 05:02
4
Extract genomic DNA using TruTip technology

Conduct automated binding, washing, and elution of genomic DNA through the porous monolithic matrix in TruTip pipette tips.

▶ 07:31
5
Assess extraction speed, fidelity, and purity

Evaluate the performance metrics of the automated extraction protocol including throughput speed, nucleic acid yield, and sample purity for clinical validation.

▶ 09:12
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