Botany JoVE (Open Access) Citable · DOI

Implantation of a New Micro Acoustic Tag in Juvenile Pacific Lamprey and American Eel

Name: Implantation of a New Micro Acoustic Tag in Juvenile Pacific Lamprey and American Eel
Uploaded: 2026-06-17
Duration: 8 min 36 s
Description: Juvenile Pacific Lamprey and American eels were used for laboratory evaluations to determine potential effects from tag implantation. Telemetry technology ...

DOI: 10.3791/59274-v

What you'll learn

✓ Prepare juvenile lamprey and eel for micro acoustic tag implantation
✓ Execute surgical implantation of acoustic tags with minimal tissue trauma
✓ Assess tag retention and post-operative fish survival outcomes

Protocol

This article illustrates the procedure for implanting a micro acoustic tag in juvenile Pacific Lamprey and American Eel. Our application is based on laboratory trials, which indicate that acoustic tags can be successfully implanted with no apparent effects on swimming ability or survival and minimal tag loss.

Difficulty

advanced

Total time

~20–30 min per fish (including anesthesia, surgery, and recovery)

Model organism

Juvenile Pacific Lamprey (Entosphenus tridentatus), Juvenile American Eel (Anguilla rostrata)

Steps

Prepare materials and anesthetize lamprey or eel

Set up surgical workspace, anesthetic solution, and micro acoustic tag equipment. Anesthetize the juvenile fish to immobility prior to implantation.

▶ 01:26

Implant micro acoustic tag into fish body cavity

Create a small incision and insert the sterilized acoustic tag into the peritoneal cavity. Close the incision with sutures or tissue adhesive to minimize infection and tag loss.

▶ 03:22

Monitor tag retention and fish recovery

Document post-operative survival, swimming ability, and tag retention rates over the monitoring period. Representative results show successful implantation with minimal adverse effects.

▶ 06:21

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