Home › Analytical Chem › In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography
Analytical Chem JoVE (Open Access) Citable · DOI

In Vivo Quantitative Assessment of Myocardial Structure, Function, Perfusion and Viability Using Cardiac Micro-computed Tomography

DOI: 10.3791/53603-v
What you'll learn
  • ✓ Apply Quantum MicroCT imaging to assess cardiac morphology and function in vivo
  • ✓ Quantify myocardial perfusion and viability using iodinated contrast agents
  • ✓ Implement longitudinal non-destructive imaging in ischemic heart disease models
Protocol

This method outlines the use of Quantum Micro-Computed Tomography (MicroCT) to assess cardiac morphology, function, perfusion, metabolism and viability with iodinated contrast agent in mice with experimentally-induced myocardial ischemia. The technique can be applied for non-destructive high-throughput longitudinal in vivo imaging of various animal models of human heart disease.

Difficulty
advanced
Total time
~2–3 hours per mouse (including anesthesia, surgery, imaging, and recovery)
Model organism
Mouse (ischemia model, species not specified)
Biosafety
BSL-1

Steps

1
Prepare cardiac ischemic model in mice

Establish experimentally-induced myocardial ischemia in mouse subjects. This foundational step creates the diseased state for subsequent imaging and assessment.

▶ 01:09
2
Perform Quantum MicroCT cardiac imaging protocol

Execute high-resolution micro-computed tomography scanning with iodinated contrast agent to acquire detailed cardiac morphology, function, perfusion, and metabolic data in vivo.

▶ 02:38
3
Analyze myocardial perfusion and viability imaging

Process and interpret MicroCT reconstructions to quantify myocardial structure, perfusion patterns, and tissue viability in ischemic and control regions.

▶ 05:45
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