Cell Biology JoVE (Open Access) Citable · DOI

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Name: Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers
Uploaded: 2026-08-05
Duration: 11 min 34 s
Description: M (microfold) cells of the intestine function to transport antigen from the apical lumen to the underlying Peyer’s patches and lamina propria where ...

DOI: 10.3791/59894-v

What you'll learn

✓ Differentiate M cells in ileal enteroid monolayers
✓ Assess M cell differentiation via immunofluorescence
✓ Maintain human stem cell-derived ileal monolayers
✓ Identify M cell markers in monolayers

Protocol

Biopharma Insights This protocol describes how to induce the differentiation of M cells in human stem cell-derived ileal monolayers and methods to assess their development.

Difficulty

intermediate

Total time

~2 weeks for differentiation plus staining

Model organism

Human stem cell-derived ileal enteroids

Biosafety

BSL-1

Steps

Induce M cell differentiation in monolayers

Treat the human ileal enteroid-derived monolayers with factors to trigger M cell differentiation.

▶ 00:27

Verify M cell differentiation

Perform assays to confirm that the cells have differentiated into M cells.

▶ 05:46

Examine immunofluorescence results

Observe and interpret the immunofluorescence staining of M cells in the monolayers.

▶ 09:30

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