Home › Analytical Chem › Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System
Analytical Chem JoVE (Open Access) Citable · DOI

Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System

DOI: 10.3791/52261-v
What you'll learn
  • ✓ Perform intubation-mediated intratracheal instillation in mice
  • ✓ Deliver reagents directly to lungs with high reproducibility
  • ✓ Characterize lung-specific delivery and assess instillation success
  • ✓ Apply IMIT for respiratory disease modeling and preclinical testing
Protocol

Biopharma Insights Intubation-mediated intratracheal (IMIT) instillation of reagents is an excellent, noninvasive method for studying respiratory disease, as well as a method for instilling therapeutic reagents directly into the lung. It is a rapid and highly reproducible method which is suitable for preclinical testing.

Difficulty
intermediate
Total time
~15–20 min per animal (including anesthesia, intubation, recovery)
Model organism
Mouse (unspecified strain)
Biosafety
BSL-2

Steps

1
Understand IMIT instillation procedure and instrumentation

Overview of intubation-mediated intratracheal instillation as a noninvasive lung-delivery method suitable for respiratory disease studies and therapeutic agent administration.

▶ 02:09
2
Perform mouse intubation and reagent delivery

Execute the IMIT protocol including animal anesthesia, endotracheal intubation, and rapid instillation of therapeutic or research reagents directly into the lung.

▶ 02:09
3
Characterize delivery efficiency and lung distribution

Assess reproducibility and verify proper lung-specific deposition of instilled reagents using imaging or biological markers.

▶ 04:52
4
Evaluate bacterial instillation outcomes in mouse lung

Review representative results demonstrating successful delivery and localization of bacterial agents to the respiratory tract.

▶ 06:03
💬 Comments coming soon