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Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Name: Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
Uploaded: 2026-10-13
Duration: 10 min 12 s
Description: Learn to isolate primary mouse hepatocytes using collagenase perfusion and culture them in 3D collagen sandwiches. This method enables long-term study of bile canalicular formation and cytoskeletal dynamics in vitro.

DOI: 10.3791/60507-v

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