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Cell Biology JoVE (Open Access) Citable · DOI

Isolation of Mouse Peritoneal Cavity Cells

DOI: 10.3791/1488-v
What you'll learn
  • ✓ Isolate mouse peritoneal cavity cells using established protocols
  • ✓ Identify and characterize immune cell populations from peritoneum
  • ✓ Prepare cells for biochemical and functional analyses
Protocol

The peritoneal cavity in mammals contains different immune cell populations crucial for innate immune responses. An efficient isolation method is required for biochemical and functional analyses of these cells. Here we provide a comprehensive method for the isolation of peritoneal cavity cells in the mouse.

Difficulty
intermediate
Total time
~30-45 min per mouse
Model organism
Mouse (strain unspecified)
Biosafety
BSL-1

Steps

1
Understand peritoneal cavity immune cell composition

Review the role of peritoneal cavity as a source of diverse innate immune cell populations and the importance of efficient isolation for downstream analyses.

▶ 00:41
2
Perform peritoneal cavity cell isolation procedure

Execute the comprehensive isolation method for extracting peritoneal cavity cells from mouse, including lavage and cell collection techniques.

▶ 01:10
3
Characterize isolated peritoneal cavity cell population

Verify the composition and phenotype of the resulting cell population to confirm successful isolation of diverse immune cell subsets.

▶ 03:35
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