Home › Microbiology › Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
Microbiology JoVE (Open Access) Citable · DOI

Laser-Induced Fluorescence Emission (L.I.F.E.) as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats

DOI: 10.3791/60447-v
What you'll learn
  • ✓ Operate laser-induced fluorescence emission (L.I.F.E.) device for in situ biomarker measurement
  • ✓ Assess phototrophic potential in supraglacial cryospheric environments
  • ✓ Interpret high-resolution spectral and spatial fluorescence data from ice habitats
  • ✓ Apply non-invasive optical methods to quantify carbon cycling in frozen ecosystems
Protocol

Biopharma Insights Carbon fluxes in the cryosphere are hardly assessed yet but are crucial regarding climate change. Here we show a novel prototype device that captures the phototrophic potential in supraglacial environments based on laser-induced fluorescence emission (L.I.F.E.) technology offering high spectral and spatial resolution data under in situ conditions.

Difficulty
advanced
Total time
~3–4 hours per field deployment site (variable with environmental conditions and measurement density)

Steps

1
Set up L.I.F.E. prototype instrument in field

Assemble and calibrate the laser-induced fluorescence emission device at the supraglacial study site. Configure spectral and spatial resolution parameters for in situ measurement.

▶ 04:35
2
Acquire fluorescence emission spectra from ice samples

Position device on supraglacial environment and capture phototrophic biomarker signals using laser excitation. Record high-resolution spectral data under ambient cryospheric conditions.

▶ 09:29
3
Analyze spectral data and validate results

Process acquired fluorescence emission data to quantify photosynthetic potential and carbon flux indicators. Compare measurements across spatial sampling locations to assess habitat heterogeneity.

▶ 12:26
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