Lateral fluid percussion (LFP), an established model of traumatic brain injury in mice, is demonstrated. LFP fulfills three major criteria for animal models: validity, reliability and clinical relevance. The procedure, consisting of surgical craniotomy, fixation of hub followed by induction of injury, resulting in focal and diffuse injuries, is described.
Total time
~1.5–2 hours per mouse (including surgery and injury induction)
Model organism
Mouse (strain not specified)
Steps
1
Perform surgical craniotomy and hub fixation
Execute surgical craniotomy to expose the brain and affix the fluid percussion hub to the skull. This prepares the animal for controlled injury induction.
▶ 01:23
2
Induce traumatic brain injury via fluid percussion
Deliver controlled fluid percussion pulse to create focal and diffuse brain injuries according to the LFP protocol parameters.
▶ 05:46
3
Assess cognitive, motor, and histological outcomes
Evaluate post-injury recovery through cognitive and motor behavioral testing, followed by histological analysis of brain tissue to characterize injury extent.
▶ 08:19