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Cell Biology JoVE (Open Access) Citable · DOI

Measuring Blood Pressure in Mice using Volume Pressure Recording, a Tail-cuff Method

DOI: 10.3791/1291-v
What you'll learn
  • ✓ Operate CODA 8-channel non-invasive blood pressure system for simultaneous mouse measurements
  • ✓ Prepare mice and equipment for Volume Pressure Recording tail-cuff measurement
  • ✓ Collect and process blood pressure data using VPR methodology
Protocol

The CODA 8-Channel High Throughput Non-Invasive Blood Pressure system measures the blood pressure in up to 8 mice or rats simultaneously. This tail-cuff system uses Volume Pressure Recording (VPR) to measure the blood pressure by determining the tail blood volume.

Difficulty
beginner
Total time
~10-15 min per measurement cycle (8 mice simultaneous)
Model organism
Mouse, Rat

Steps

1
Understand Volume Pressure Recording blood pressure measurement

Review the principles of non-invasive tail-cuff measurement and how VPR technology determines blood pressure by monitoring tail blood volume changes.

▶ 00:41
2
Set up CODA system hardware and software

Configure the 8-channel CODA device, connect sensor pods, and initialize measurement software for simultaneous animal monitoring.

▶ 01:13
3
Acclimate and position mice for measurement

Place mice in individual warming chambers and fit tail-cuff sensors on each animal to ensure proper contact and baseline acclimation.

▶ 04:34
4
Execute data collection and process results

Run the automated measurement protocol to simultaneously acquire blood pressure readings from up to 8 animals and export processed data.

▶ 07:06
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