Home›Cell Biology›Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
Cell BiologyJoVE (Open Access)Citable · DOI
Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
DOI: 10.3791/50254-v
What you'll learn
✓Mount optical setup for scattering measurements
✓Capture images under varied incident angles
✓Process images to extract scattering directions
✓Visualize scattering at biological sampling locations
Protocol
Biopharma Insights We present a non-destructive method for sampling spatial variation in the direction of light scattered from structurally complex materials. By keeping the material intact, we preserve gross-scale scattering behavior, while concurrently capturing fine-scale directional contributions with high-resolution imaging. Results are visualized in software at biologically-relevant positions and scales.
Difficulty
advanced
Total time
~2-4 hrs per sample
Steps
1
Mount and prepare sample for imaging
Position the object, light source, and camera to enable controlled illumination and imaging.
▶ 01:42
2
Acquire and process sparse incident direction measurements
Collect images from a sparse sphere of incident directions and process them to extract scattering data.
▶ 02:55
3
Browse and visualize scattering measurements
Use software to explore and visualize the measured light scattering from the sample.
▶ 03:43
4
Prepare for multiple camera positions
Adjust the setup to allow measurements from multiple camera positions.